A Study Of The Effect Of Polycyclic Aromatic Hydrocarbon On Polymorphisms Gstp1 Gene On Thi-Qar Refinery Oil Workers

Abstract

The influence of occupational exposure of chemical compounds on the detoxification gene GSTP1 in tar workers since these chemical compounds which have multiples effects. Therefore, the present study aimed to determining the influence of PAH and the role of GSTP1 gene according to some criteria (gender, age, smoking, location and family history). The current study included 50 workers with highest GSTP1 lacking of workers 48%compare with control 40%at the level of significant (P≤0.05). DNA was Extracted from the two sets and amplified GSTM1 gene with of technology of Polymerase Chain Reaction (PCR) so as Albumin gene (internal control). DNA was Extracted from the two sets and amplified GSTP1 gene with of technology of Polymerase Chain Reaction (PCR) so as Albumin gene (internal control). The higher percentage of GSTP1 lacking in workers (40%) compare with healthy controlled group (24%) at the level of significant (P≤0.05). Current study showed also not worthy that long work period in benzene and Tetra Ethyl Lead had an obvious lacking due to mutation, causing to delete that genes about ten times compare with others chemical compounds. When study samples are divided into old workers and new one, the results showed That old are subject to gene deletion more Than the new ones.