Isolation And Identification Of Paenibacillus Bacteria By Biochemical Tests And Molecular Techniques

Abstract

The current study aims to isolate and diagnose the bacteria Paenibacillus from different types of local yogurt in the city of Mosul. (80) Yogurt samples were collected, (20) which constitute of each of (sheep, cows, buffaloes and goats), 10 isolates of Paenibacillus were obtained from them. (12.5%) of the total population of the samples under study, the bacterial isolates were distributed in to (5) isolates from sheep’s yogurt, two isolates from cow’s yogurt, and (3) isolates from buffalo yogurt with percentages of (50%, 20%, 30%), respectively, However, no isolate was obtained from goat's yogurt. Bacterial isolates were diagnosed based on morphological and biochemical tests, diagnosis was confirmed by polymerase chain reaction (PCR) using the 16S rRNA gene of the bacteria under study. In order to decide on the strain type and the isolates, the bacterial isolates that were undergone PCR test, were sent to Macrogen biotechnology company affiliated to the Japanese Hitachi company using the Genetic analysis 3130 device, the sequence of nitrogenous bases of the 16S rRNA gene of Paenibacillus was identified, and after comparing it with its globally classified analogues on the NCBI website, (3) different types of Paenibacillus were diagnosed, two of which were diagnosed and registered in the genebank as Paenibacillus lautus and Paenibacillus ginsengagri, and the third type was diagnosed and registered as a new local isolate in the genebank within the current study under the name Paenibacillus sp.RAN.