A Novel Quantitative Method For The Validation Of The Dosage Of Amnesic Shellfish Poisoning Toxins In Bivalve Mollusks

Abstract

The intra-laboratory validation of an analytical method is defined as the action consisting in subjecting an
analytical method to an intra-laboratory statistical study, based on a standardized and/or recognized
protocol, and in providing proof that in its field of application, the analytical method meets the pre-
established performance criteria. In this study, we used the DR-12-VMC protocol (Rev. 2012) and the Quilliam,
M.A. protocol for the validation of a method of assaying toxins, in bivalve mollusks used internally, causing
amnesic poisoning by mollusks (ASP). Thus, we proceeded to characterize the performance of the analytical
method used by evaluating, through experimental analysis and statistical tests, several parameters among the
most frequently recommended, namely: limit of detection, limit of quantification, compliance rate, linearity,
precision, sensitivity, specificity, accuracy, and recovery.