Tet (B) Genedetection Of P. Aeruginosaande. Coli In Burn Patients And Urinary Tract Infection In Thi-Qar Province/ Iraq

Abstract

The goal of the present study was to profile the tet (B) gene coded to resistance of tetracycline antibiotic via PCR technique in P. aeruginosa andE. coli isolates which isolated from55 swabs of burn patients and 60 urine samples of patients with Urinary Tract InfectionAl-Hussein Teaching Hospital in Thi-Qar province/ Iraq during the period from March to November, 2020. Only 58.33% isolates were identified asE. coli afterward identified by morphological and biochemical tests. Whereas 43.7% of isolates identified as P. aeruginosa from all burn samples subsequently diagnosis by morphological characteristic on culture media and API20 System. The molecular results showed that the tet (B) genewas found in 93% and 96% of P. aeruginosa andE. coli isolates, respectively.