3146 Determination Of The Genetic Relationships Among Five Species From Families Boraginaceae,Papaveraceae, Plantaginaceae And Polygonaceaeby Rapd And Issr Analysis In Iraq

Abstract

Isolation of the genomic DNA from leaves of the species under study belonging to different families with concentrations ranging between (70-163ng/ml) and purity of (1.75-2) which was estimated by a Nanodropdevice at the wavelengths of 260 - 280 nm, Random Amplified Polymorphic DNA (RAPD) and The Inter-simple sequence repeat (ISSR).The discrepancies between the replicated pieces of each species (their numbers and molecular sizes) were detected after the replication products of the samples were migrated onto the agarose gel and stained. With the stain of Red safe staining solution,(20) selected primers showing varied outcomes of multiplication among the species examined, as those primers showed with (RAPD) Marker (189) polymorphic bands out of (197) total main bands, While with (ISSR) Marker (52) polymorphic bands out of (56) total main bands.
For RAPD-PCR analysisit gave the primers OP-E20, OP-L05, OP-L20 and OP-M05 and OP-V19 unique fingerprints for each genotype, but this did not apply to other primers.While, ISSR- PCR analysisthe primers UBC842 and A35, UBC480, and A34 are uniquely fingerprinted for each genotype, but this did not apply to other primers.
These methods were good in diagnosing the species understudy