Optimization Of Fermentation Conditions For Enhanced Production Of Secondary Metabolite From Endophytic Fungi Of Berberis Aristata

Abstract

Purpose: Berberis aristata, an excellent herb mentioned in Ayurveda since ancient times, is found in the Himalayan regions of India. Almost every part of the herb is used in treating various diseases since ancient times. The roots and leaves of Berberis aristata have high medicinal value and are used to treat eye-diseases, osteoporosis, diarrhoea, diabetes, cancer, gynaecological disorders, etc. Any organism (bacterial or fungal endophytes) isolated from this species can be of immense interest due to its potential of producing novel bioactive compounds.
Methods: In this study, we isolated 10 fungal endophytes which were identified through morphological and internal transcribed spacer (ITS) sequence analysis. Diversification of fungal endophytes and species richness was higher in leaf tissues as compared to root segments sampled for isolation. Antibacterial activity was observed by the ethyl extract of 80 % fungal isolates against one or more bacterial pathogens. Further the fungal endophytes were optimized for growth and production of antimicrobial compound in batch culture system. PDA media supplemented with 0.1g chloramphenicol favoured the growth at temperature of 30℃ and incubation period of 6-7 days.
Results: We are reporting for the first time Talaromyces flavus as fungal endophyte of Berberisaristata producing berberine.
Conclusion: This study can prove possible capability of endophytes for the production of plant metabolites having antimicrobial potential for which medicinal plants are regularly exploited and becoming endangered thus by such studies we can explore new antimicrobial agent from endophytes and at the same time conserving our biodiversity.