Synthesis And Characterization Of Silver Nanoparticle From Cyphostemma Auriculatum Roxb.: Its Application As Antibacterial Activity And Mode Of Action

Abstract

This investigation demonstrated the accurate reduction and stability of silver nanoparticles (AgNPs) using ethanolic dried Cyphostemma auriculatum Roxb. leaf extract as an effective reducing and stabilizing agent. The presence of AgNPs in the colloidal solution was confirmed by the highest absorption peak at 409nm in UV-Vis spectroscopy. According to FTIR significant vibrational spectra obtained from the leaf extract, Cyphostemma auriculatum Roxb.leaf extract proteins were primarily responsible for the reduction and stabilization of AgNPs. X-ray diffraction studies were employed to determine the crystal structure of metallic AgNPs. The average particle diameter was calculated using the Debye-Scherrer equation. The SEM images show AgNPs with a rough surface topology and nanoscale dimensions. The researchers created spherical, polydistributed, and monoclinic silver nanoparticles using Cyphostemma auriculatum Roxb.(CA-AgNPs) with an average diameter of 15 nm. Staphylococcus aureus MTCC 96, Micrococcus luteus MTCC 2470, Klebsiella planticola MTCC 530, and Escherichia coli MTCC 739 had minimum inhibitory concentrations (MICs) of 0.9, 3.9, 1.9, and 1.9 μg/mL, respectively. For the same bacteria, the minimum bactericidal concentration (MBC) values are 1.9, 7.8, 3.9, and 3.9 μg/mL, respectively. The biofilm-inhibiting activity of the CA-AgNPs found to have antibacterial activity in this study was evaluated. Micrococcus luteus MTCC 2470 was the most effective at biofilm suppression, with an IC50 value of 29.84 g/mL. According to the findings of the cytoplasmic leakage investigation, CA-AgNPs may have caused bacterial strains to rupture their membranes. When the cytoplasmic membrane's permeability is compromised, internal components, primarily potassium ions, leak out. Antibacterial experiments involving ROS formation revealed that oxidative stress in bacterial cells was effective at achieving antibacterial effects. In this study, silver nanoparticles (CA-AgNPs) derived from Cyphostemma auriculatum Roxb. green could be used as medicinal agents.