Impact Of Oxidative Stress On Level Of Biomarkers Associated To Type 1 Diabetes

Abstract

Type1 diabetes mellitus (T1DM) is characterized by hyperglycaemia since insulin secretion is inhibited by pancreatic islets. Oxidative stress is a generally recognized participant in the incidence and development of T1DM and its complications. The study aims to investigate the effect of oxidative stress including H2O2 and NO on the biomarkers related to the diagnosis of T1DM disease. The study has involved 80 participants within age (5-25 years); 50 T1DM patients (24 boys and 26 girls) and 30 healthy volunteers (18 boys and 12 girls).The level of fasting blood glucose (FBG), glycated haemoglobin (HbA1c),lipid profile (total cholesterol, triglyceride, LDL, and HDL), liver function test (AST, ALT), renal function test (B.urea, S.creatinine), Ca+2,Fe+2,H2O2 and NO were determined. The data for this study were analysed and presented as mean ± standard deviation, Pearson’s correlation test was applied to examine various correlations. Two-tailed P-values were used and statistical significance has been considered as P < 0.05. The results have shown significantly higher levels (P>0.0001) of H2O2 inpatients with type1 diabetes compared to control, which is in the patient's group was (75.31±25.61), and in the healthy group was (50.51±17.00). However, there were non-significant differences in the levels of NO between Type1 diabetic patients and the healthy group which is (36.05±20.86μmol/L),(32.18±8.83 μmol/L) respectively. Pearson’s correlation test has shown no correlations between oxidant markers and biomarkers. As a result, it can be concluded that the levels of oxidative stress such as H2O2 and NO have not a direct impact on the level of other biomarkers related to the diagnosis of T1DM.