Isolation, Screening And Optimization Of Amylase Producing Bacteria From Soil In Dairy Farm Sarband Peshawar, Kpk, Pakistan

Abstract

Microbial enzymes have a variety of characteristics that make them valuable in a wide range of applications. Amylases are enzymes that are responsible for the breakdown of starch or glycogen. According to the findings of this study, amylase-producing bacteria isolated from soil samples of Dairy Farm Sarband Peshawar, KPK, Pakistan, were screened and characterised. As a result, ten bacterial isolates were recovered from the collected soil samples. This group selected two isolates (U5 and U6) based on the average clear zone development diameter and the time required to decolourize iodine solution, respectively. Based on their morphological and biochemical features, the isolates belonged to the genera Bacillus and Staphylococcus. The optimal amylase production time for the (U5) isolates was 72 hours, and for the (U6) isolates, it was 24 hours. This corresponded to amylase activity of 15mm zone of hydrolysis for U5 and 11mm zone of hydrolysis for U6, respectively, for the isolates. Studies on the influence of pH showed that the crude enzyme with the highest activity and stability was found at pH 9 and 7 for isolates U5 and U6, respectively. This amylase-producing bacterial strain has a lot of promise for use in Pakistan's food and agricultural industries, and it has a lot of potential.