Phytochemical Investigation And Anti-Diabetic Activity Study Of The Plant Of Cleome Rutidosperma

Abstract

The hypoglycaemic and antihyperglycaemic effects of metha-nol extract of leaves of Cleome
rutidosperma (Cr) DC (Family: Capparidaceae) was investigated in Wistar rats. Fifty normoglycaemic male rats (120 g–200 g) were divided into groups A (hypoglycaemic study; n = 20)
and B (antihyperglycaemic study; n = 30). Each experiment had one control group and three
groups administered with Cr (100, 200 or 400 mg/kg) respectively. Group B had two additional
groups of diabetic-untreated rats and glibenclamide-treated diabetic rats. Diabe-tes was induced
in Group B rats (except control) fasted over-night for 12 h by intraperitoneal injection of Alloxan
(100 mg/kg). Fasting blood glucose levels (FBGL) were determined and alloxan-treated rats with
BGL > 200 mg/dl 48 h post-induction were considered diabetic. Data obtained were analyzed
using One-way ANOVA and Duncan Multiple Range Test (p < 0.05). Cr-treated rats showed
significant decline in BGL with notewor-thy decline by day 3 post-treatment at the dose of 200
mg/kg (236.40 ± 14.72 mg/dl) from 336.40 ± 21.06 mg/dl. Cr at the dose of 200 mg/kg (72.20 ±
6.18 mg/dl, 69.20 ± 7.81 mg/dl, 137.80 ± 7.15 mg/dl and 70.60 ± 10.66 mg/dl) showed better gl
ycemic control compared to glibenclamide (194.50 ± 7.75 mg/dl, 253.75 ± 7.20 mg/dl, 284.25 ±
10.56 mg/dl and 156.00 ± 10.80 mg/dl). Cr-treated rats also showed pro-gressive weight gain
through the course of the study. This study demonstrated Cr has antihyperglycemic effect with
more rapid onset of action and better glycemic control compared to glibenclamide