Histopathological Alterations In The Stomach Of Rats After Gavage Ethanol

Abstract

The study aims to detect the effects of ethanol on pathological changes in the stomach according to the fed and fasting states
and detect whether interferon-gamma (IFN-γ) represents an indicator measuring the impact of ethanol on induced damage
grades. Twenty-five adult albino rats with average weight (230 ± 12) g, divided into five groups (5 in each), groups (A, B), and
groups (C, D) administered with (30%, 50% ethanol ) respectively in fed and fasting state, and group E served as the control
group. The treated groups administered (1 ml) ethanol orally every 48 hours for an entire month. at the end of the experiment,
the rats anesthetized and the stomach fixed in formalin used for routine histological procedures, the serum samples obtained
from blood were used to measure the level of IFN-γ by enzyme-linked Immunosorbent Assay (ELISA). The histological analysis
of treated groups with 30% ethanol reveals a mild alteration in gastric mucosa and more change appeared at 50% ethanol.
These changes appear more severely in the fasting state compared to the fed status for the same concentration. Results reveal
a significant increase (P<0.05) in serum level of IFN-γ in the treated groups with increasing ethanol concentration and the
fasting state. It can conclude that the severity of the damage in the gastric mucosa and the serum level of IFN-γ increase with
increasing the concentration of ethanol and the fasting state.