Micropropagation Of Paulownia Tomentosa (Thumb. Stud.) By Tissue Culture

Abstract

Paulownia trees are adapted to grow in different environments, where they grow in the tropics and temperate climates and
withstand temperatures of 25-47 ° C. Given the importance of the plant and its widespread, this study was conducted in the
Laboratory of Textile Agriculture at the College of Agriculture, University of Basra for the year 2020.To study the testing of
different levels of plant growth regulators (auxin and cytokinin) in stimulating the formation of the primary callus tissue, the
formation of for adventitious shoots from it, their multiplication, the formation of vegetative shoots, their rooting and their
acclimatization. The results of the study showed Callus tissue was formed when auxin NAA at a concentration of 1 mg.L
-1
and cytokinin TDZ at a concentration of 5 mg.L
-1 were used with the least time period of 20 days and the highest fresh weight
of 2.94 g and dry weight of 0.36 g in the MS medium with full strength, It was observed that the use of auxin NAA at a
concentration of 0.5 mg.L
-1 and cytokinin TDZ at a concentration of 2 mg.L
-1 gave the shortest period of time for adventitious
shoots to be 20 days and numbering 8.66 shoots .The use of auxin NAA at a concentration of 1 mg.L
-1 and cytokinin TDZ at
a concentration of 5 mg.L
-1
resulted in the highest shoots multiply average of 22.3 shoots and an average length of 7.3 cm,
The use of auxin IBA at a concentration of 1 mg.L-1
resulted in the formation of roots with the highest average of 12 roots
and a length of 6.6 cm, It was noted that the percentage of adapted plants increased to 90-100% than irrigation with MS
salts at half and a quarter of the strength, respectively. The highest content of total chlorophyll pigment was 38.96%,
carbohydrate content was 35.5 mg.g-1
, and the average of proline was 1.96 mg.g-1