Differentiation Of M. Tuberculosis Complex By PCR In Clinical Samples

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Ada O. del Carpio-Sanz , Patricia Del Portillo , Hugo J. Rojas-Flores Amalfi , R. Gallegos-Morante , Luis C. Lizárraga-Vargas , Víctor G. Torres-Diaz


Tuberculosis, caused by microorganisms of the so-called Mycobacterium tuberculosis complex (MTBC),
continues to be a public health problem worldwide, as MTBC diverged into different lineages, gene expression
of virulence y associated metabolites also did, this provides clear evidence that MTBC lineages likely reflect
adaptation to different human populations.
The difficulties to manipulate mycobacteria, their slow growth and pathogenicity have made them a difficult
model to genetically characterize. However, the development of tools for genetic manipulation has allowed
advances in research, facilitating the understanding of genome organization, gene expression and phenotypic
determination, which explain the mechanisms of pathogenicity, latency and drug resistance.
This paper reports the differentiation of the CMTB by Polymerase Chain Reaction (PCR) in samples from
patients with clinical diagnosis of tuberculosis, from different genomic regions of the microorganisms of the
complex in samples from patients with clinical diagnosis of tuberculosis. For the development of the present
study, sputum samples were collected from patients with a microbiological diagnosis of tuberculosis, obtained
from two hospitals in Arequipa.
The extraction and purification of DNA from clinical samples was carried out and then amplified by PCR. and
identified PCR, for which a group of different DNA amplifiers were used. Initiators which amplifies the DNAn
depending on the type of MTBC, allowing the results identified the Mycobacterium tuberculosis strains with
73%, followed by Mycobacterium of unidentified Mycobacterium with 20% and 2% of Mycobacterium of
Mycobacterium canetti, strains that circulate in the region.

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