Efficiency Of Pseudomonas Aeruginosa In Bioremediation Of Chlorpyrifos Toxicity

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Hassanin Sabah Hashim , Ameera O. Hussain Al-Janabi and Amer O. Saud AlShammari

Abstract

At present study, extensive varieties of pesticides are being used but the demand for Organophosphorus
pesticide is increasing globally to control insects. Chlorpyrifos is a broad-spectrum, highly toxic, and
chlorinated organophosphate insecticide that is synthetic in origin and is normally ester or thiol
derivatives of phosphoric. The mode of action involves inhibiting acetyl-cholinesterase leading to the
accumulation of acetylcholine causing neurotoxicity. It is being transported by circulation far away from
the site of application leading to pollution of the environment due to its persistence, it is not only severely
detrimental to the target pests but also causes toxicity in non-target organisms including humans. Lately,
research activities in this area have demonstrated that microorganisms are potential tool in decaying
insecticides into less harmful and non-toxic metabolites through a process known as bioremediation.
Bioremediation has now emerged as an innovative technology that is critically important for the clean-up
of polluted sites. This study aimed at investigating the efficiency of pseudomonas aeruginosa in
bioremediation of chlorpyrifos from contaminated soil in Iraq. HPLC technology was used to estimate the
percentage of removal achieved by bacteria, and it is considered one of the advanced modern techniques
in measuring ingredient active in solutions. In concluded from the present study the results of the HPLC
analysis showed that Pseudomonas aeruginosa is highly efficient in the analysis of chlorpyrifos.

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