Isolation And Identification Of Potentially Pathogenic Bacteria From Second Hand Items Of Flea Market Of District Haripur, Pakistan

Abstract

Secondhand is one of the sources of illness owing to disease and harm caused by the environment. Used clothing is sold all around the country since it is much cheaper than new clothing. The current investigation involved collecting samples from various used clothing market places in Haripur, Pakistan, and comparing the efficacy of detergent used to reduce microbial load in the garments, as well as checking antibiotic susceptibility for the isolated bacterial strains. Adult and baby clothing, fabric toys, underwear, bags, and bed covers were among the samples. The identification was done using culture and biochemical methods. In adult garments, Staphylococcus aureus was the most common isolate bacteria, followed by Bacillus subtilus in baby clothes and fabric toys. The highest levels of CFU were identified in baby garments (4x106), fabric toys (2.2x104), and adult clothes (20x102), respectively. Furthermore, these bacteria have shown resistance to the majority of antibiotics. These resistant strains may cause serious sickness in those who buy used goods. PCR was done to confirm the presence mec A gene responsible for Methicillin resistance.