Antibacterial Activity Of Bacterial Mediated Synthesized Iron Oxide Nanoparticles Using Bacillus Coagulans

Abstract

Cells free supernatant of Bacillus coagulans gram positive, spore forming, bacteria grown in brain heart
infusion broth medium (BHIB), was employed as a stabilizing and bio reducing agents to synthesize Iron
Oxide Nanoparticles (IONPs) .This method was appeared as an alternative to conventional chemical and
physical methods. The synthesized (IONPs) were characterized with Ultraviolet-visible spectroscopy (UvVis), X-ray diffraction (XRD).and scanning electron microscopy (SEM) . The rapid produced nanoparticles
resulting in small average particles size 15.18 nm. The IONPs was experimented as antibacterial agent
against Uropathogenic E. coli at concentration of 100, 200, 300, 400 µg/ml, the result showed IONPs was
effective at concentrated depending manner and it become more effective when the concentrated was
increased .