Evaluation Of Antimicrobial Activity And Characterization Of Silver Nanoparticles By Bacterial Isolate

Abstract

Silver nanoparticles revealead to be more powerful tool against Gram-positive and Gram-negative bacteria with
bactericidal activity together with multi resistant strains. The cell supernatant of E.coli species was used in this study
for the silver nanoparticles synthesis. The synthesized Ag NPs optical density was measured in different cell
supernatant. The multi drug resistant pathogen E.coli was chosen due to its highest Ag NP synthesis. The
spectrophotometric analysis of Ag NPs at the band ƛ 431 nm for characteristic Surface Plasmon Resonance indicated
that Ag nanoparticles are in spherical or roughly spherical shaped. FT-IR spectrum of Ag NPs observed at 1359.35
cm–1, 1350.3 cm-1 and 1398.40cm-1. The Ag NPs synthesis showed the antimicrobial activity for the E.coli measured
was 18.67±2.082 diameter in zone on inhibition. The Ag NPs synthesized values of MIC against the tested clinical
pathogen showed the antimicrobial activity at 200 ppm for E.coli respectively.