Synthesis And Optimization Of Silver Nanoparticles By Bacterial Isolate E.Coli Spp

Abstract

The use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach. In
the present study, we observed the synthesis of silver nanoparticles and we reported the biosynthesis of silver
nanoparticles employing the bacterium E.coli. The multi drug resistant pathogen E.coli was chosen due to its highest
Ag NP synthesis. The higher level of nitrate reductase activity was measured at 731.24 U/ml in the 60th run with
presence of (%) glucose: 0.1%, peptone: 1%, yeast extract: 0.4%, KNO3: 0.4%, pH: 7.5 at 25 ˚C and 3 days incubation
period. AgNO3 showed more effective treated with gamma radiation of after mixing than before mixing with silver
nitrate solution.