Evaluation Of Anti-Diabetic And Wound Healing Potential Of Ethiopia Plant ‘Ruta Graveolens’ In Diabetic Induced Rat

Abstract

Ruta graveolens L. (Rutaceae), locally known as ''tena adame'', originally native to the Mediterranean region and is
widely distributed in various geographical regions of Ethiopia. It is ornamental and medicinal plant used
traditionally. The present study was carried out for the phytochemical analysis and to evaluate the anti-diabetic
and diabetic induced wound healing activity of acetone, aqueous and chloroform leaf extract of Ruta graveolens
(RG). Preliminary screening of the extracts result shows the presence of various phyto-chemical compounds.
Wistar albino rats (200-250gm) were used as experimental animals and were divided into six groups. Group I, as
the control group, received simple ointment (Vaseline) and 4mg/kg /sc dexamethasone, Group II tetracycline
hydrochloride ointment plus 4mg/kg /sc Dexamethasone (Dexa). Group III, IV, V&VI received Glibenclamide
(GLB)5mg/kg, 20% ointment of acetone extract (AE), aqueous extract (AQE) and chloroform extract (CE) along with
4mg/kg/sc dexamethasone respectively. Phyto chemical screening, wound contraction and blood glucose level was
monitored on Days 0, 2, 4, 6, 8, and 10. Result showed, the chloroform extract in treated animals showed a
significant result in decrease glucose in blood, period of epithelization and wound contraction in diabetic rats.
Study concluded that chloroform extract comparatively shows good diabetic and wound healing property.