Non Anthrax Bacillus Species Association In Non Healing Diabetic Foot Ulcers And Their Drug Susceptibility

Abstract

INTRODUCTION
Non-anthracis Bacillus species are ubiquitous gram-positive spore-forming organisms that were once believed to be non pathogenic
but are now recognized as causing a variety of infections.A Diabetic foot Ulcer is an open wound in the skin that does not heal within
days or weeks and people with diabetes are at even more risk of developing diabetic foot ulcers and pain.Diabetic Foot ulcers are
open sores or lesions that will not heal or that return over a long period of time.
AIM
This study is done to find out the association of Non anthrax Bacillus species in the non healing diabetic foot ulcer
OBJECTIVE
To know the significance of the presence of Non anthrax Bacillus species in diabetic ulcers.To know about the specific treatment
outcome and the drug of choice
MATERIALS AND METHODS
Study is done in patients visiting the diabetic clinic OP department. Samples were collected from patients who are clinically proven
diabetic and having non healing foot ulcers and elected for medical management. Swabs samples were collected and transported to
the department of microbiology. Samples were stored at 4 deg.C till it was processed. Samples were then inoculated on Blood agar,
Nutrient agar and MacConkey agar and incubated at 37 deg.C for 24 hours. The growth on Macconkey agar was identified by gram's
staining for the presence of gram positive bacilli with spore and for chain formation. Those colonies are biochemically identified as
Bacillus species and tested for their drug susceptibility. The most common species identified is Bacillus cereus. The results were
tabulated and analysed.
Results
In this study a total number of 82 samples were collected from foot ulcers in diabetic patients. The samples were subjected for
microbiological examination. In this 13 bacterial isolates were identified and the proportion of Bacillus sps association is determined.
Bacillus sps were isolated from 8 patients which was the fourth highest in number of the bacterial sps isolated. Bacillus sp is not a
common finding in a healthy patient.
Conclusion
Isolation of bacillus species in the diabetic patients should be given due importance, because it is found as a single isolate in many
diabetic patients with non healing foot ulcers.