Evaluation Of Antidiabetic And Cytotoxic Effect Of Boerhavia Diffusa Mediated Selenium Nanoparticles

Abstract

Introduction: Boerhavia diffusa is a species of flowering plants, also known as the four o'clock family commonly called Punarnava. It
is used as a herbal medicine for pain relief. Leaves of Boerhavia diffusa are used as green vegetables in most parts of the world.
Chloroform extract of root and aerial parts of Boerhavia diffusa is used in ayurveda for the treatment of diabetes mellitus. Selenium
nanoparticles play an important role in inhibition of bacterial growth at very low concentrations of protein, a high amount is required
for inhibiting bacterial growth individually. They act on both the intact and complex form of the extract. These nanoparticles serve
to reduce the toxicity, enhance bioactivity, and improve targeting.
Aim: The main objective of this study is to estimate the anti diabetic and cytotoxic activity of Boerhavia diffusa mediated through
Selenium nanoparticles.
Materials & Method: Extract preparation- 1g of Boerhavia diffusa was added in 100ml of distilled water and is boiled for 10 - 15
minutes at 70 degree Celsius.
Brine shrimp lethality Assay:Number of dead nauplii / Number of dead + Number of live nauplii x 100.
In vitro diabetic Assay: %inhibition = C-T/Cx100, where C-control & T-test sample.
Results: Boerhavia diffusa has a significant antidiabetic and cytotoxic activity. It helps in blood glucose reduction by rejuvenation of
pancreatic B cells or by extra pancreatic action, which is said to be its antidiabetic activity. From the results obtained, it is clearly seen
that Boerhavia diffusa has a major antidiabetic activity and a moderate cytotoxic activity.
Conclusion: From the study it can be concluded that Boerhavia diffusa aids in the therapeutic management of chronic ailments. It
has major anti diabetic and moderate cytotoxic activity. It helps in the treatment of diabetes and renal diseases. Further studies have
to be done on other activities of Boerhavia diffusa