Influence Of Oxidative Biomarkers On APE1 Gene Expression In Iraqi Breast Cancer Women

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Altayeb Jasim Mohammed , Murat Çanlı


This study aimed to investigate the APE1 gene expression with some clinical biomarkers in breast cancer women. Ninety
women were enrolled in this study; they were divided equally into 2 groups: the breast cancer patients’ group, which
attended the Al-Eluia hospital for women's care in Baghdad, and the healthy control women's group. Five milliliters of
blood were taken from patients and a healthy control group. Two milliliters of blood were transmitted directly to EDTA
tubes, and then the ELISA technique was used to determine the oxidative biomarkers like Glutathione Peroxidase 1
(GPX1), Estradiol (E2), Malondialdehyde (MDA), and reactive oxygen species (ROS). Three milliliters of blood were used
for gene expression by using the RT-PCR method. The result of this study showed that there were significant differences
in the expression of the APE1 gene were the gene folding for patients was 3.004 ±0.15 when compared with the control
group 1.00 ±0.00. In conclusion, when comparing breast cancer patients to healthy people, it was discovered that the
APE1 gene was out of the ordinary.

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