Influence Of Oxidative Biomarkers On APE1 Gene Expression In Iraqi Breast Cancer Women

Abstract

This study aimed to investigate the APE1 gene expression with some clinical biomarkers in breast cancer women. Ninety
women were enrolled in this study; they were divided equally into 2 groups: the breast cancer patients’ group, which
attended the Al-Eluia hospital for women's care in Baghdad, and the healthy control women's group. Five milliliters of
blood were taken from patients and a healthy control group. Two milliliters of blood were transmitted directly to EDTA
tubes, and then the ELISA technique was used to determine the oxidative biomarkers like Glutathione Peroxidase 1
(GPX1), Estradiol (E2), Malondialdehyde (MDA), and reactive oxygen species (ROS). Three milliliters of blood were used
for gene expression by using the RT-PCR method. The result of this study showed that there were significant differences
in the expression of the APE1 gene were the gene folding for patients was 3.004 ±0.15 when compared with the control
group 1.00 ±0.00. In conclusion, when comparing breast cancer patients to healthy people, it was discovered that the
APE1 gene was out of the ordinary.