Method Development And Validation For The Simultanious Estimation Of Chlordiazepoxide In Bulk And Tablet Dosage Form By Reverse Phase High Performance Liquid Chromatography

Abstract

Chromatographic parameters were optimized to develop HPLC methods for individual drugs and for
simultaneous estimation of combined dosage forms of Imipramine and Chlordiazepoxide in pharmaceutical
formulations with short analysis time with acceptable resolution. In the present study a simple, rapid, accurate
and robust HPLC method was developed for the estimation of Imipramine and Chlordiazepoxide in their
pharmaceutical formulations. The optimum detection wavelength for Imipramine and Chlordiazepoxide
combination was 255nm. The mobile phase optimized for Imipramine alone and Imipramine + Chlordiazepoxide
combination consists of Methanol and disodium hydrogen phosphate buffer of pH 3.5 in the ratio of 45:55. The
mobile phase optimized for Chlordiazepoxide alone was ammonium formate buffer of pH 3 and acetonitrile in
the ratio of 67:33 v/v. Columns finalized for individual drugs are imipramine and chlordiazepoxide Thermosil RP
C18 (4.6*150mm) 5µm were used as stationary phases. The solutions were chromatographed at a constant flow
rate of for Imipramine alone and Imipramine + Chlordiazepoxide combination the flow rate was found to be
ideal at 0.7ml/min and for Chlordiazepoxide alone the flow rate selected was 0.8ml/min. Linearity was set up
over the focus scope of 10ppm to 50ppm for Imipramine, 15ppm to 75ppm for chlordiazepoxide and Correlation
coefficient was seen as 0.999. The mean % Recovery for Imipramine bulk drug it was found to be 99.07%, 99.87%
and 100.22% respectively and for Chlordiazepoxide it was found to be 99.41%, 100.02% and 99.67% respectively
are within the limits. The mean percentage recovery for Imipramine and Chlordiazepoxide combination at 50%,
100% and 150% levels were found to be 99.64%, 100.04% and 99.89% respectively for Imipramine and 99.57%,
99.78% and 99.83% respectively for Chlordiazepoxide and are within the limits. The LOD value obtained for
Imipramine and Chlordiazepoxide combination is 2.951 for Imipramine and 2.878 for Chlordiazepoxide and the
LOQ result obtained is 9.902 for Imipramine and 9.731 for Chlordiazepoxide. The percentage purity of
Imipramine and Chlordiazepoxide combination was found to be 98.62% for Imipramine and that of
Chlordiazepoxide was found to be 99.60%