Method Development And Validation For Metal Analysis Of Herbal Drug (Abhrakbhasma) Using Inductive Coupled Plasma Optical Emission Spectroscopy

Abstract

Herbal medicines have been using for the treatment, management, and prevention of different types of diseases by majority of the population in India since time immemorial. These medicines are effective besides being easily available, and inexpensive compared with allopathic drugs. In recent times, several cases of heavy metals in herbal medicine have come to light. As a result, regulators have started monitoring for controlling the quality of herbal medicines. The present study pertains to the development of a validated method to determine the content of lead, cadmium, arsenic, and mercury in Abhrak Bhasma. Inductive coupled plasma optical emission spectroscopy was used for this purpose. The present study was performed to evaluate heavy metals contents by Inductive coupled plasma optical emission spectroscopy. Correlation coefficient was calculated from the linear regression analysis proved that the developed method obeys linearity for Abhrak Bhasma. Results for %RSD for replicates of each standard of Abhrak Bhasma was found to be in the range of 0.16 to 1.89. Results for %RSD for 3 replicates of sample solution of Abhrak Bhasma was found to be 0.16 to 2.94. The obtained %recovery for check standard of Abhrak Bhasma was found to be in the range of 91-104.50%. The results indicate that ICP-OES is an alternative method for use in the determination of heavy metals and other elements of interest in herbal medicines and for quality control of crude drugs and their products.