Miraculous Antibacterial Activity With A Synergy Between Salicylic Acid And Rosmarinus Officinalis Essential Oil Against The Multidrug Resistant Pathogen Pseudomonas Aeruginosa. In Vitro And In Silico Studies.

Abstract

Pseudomonas aeruginosa occupies a central position in the current problematic of nosocomial infections. Such infections are becoming an everyday occurrence. This dire scenario requires stringent adherence to antimicrobial stewardship and isolation of colonized or infected patients at all times.  Unfortunately, some secondary metabolites from plants, such as essential oils, phenolic compounds, and alkaloids, were shown to be ineffective against Pseudomonas aeruginosa, allowing us to test the interaction of an antibiofilm with an antibacterial drug and evaluate how effective it was.  

The purpose of this research is to see how effective Rosmarinus officinalis L essential oil alone and combined with salicylic acid is against Pseudomonas aeruginosa, on the other hand, to evaluate in silico, the inhibition of Las B elastase and Las A alkaline by the major compounds of rosemary essential oil and involved interactions for the first time between them.

  Rosemary essential oil was ineffective against hospital strains of Pseudomonas, although Pseudomonas aeruginosa ATCC7853 was sensitive to the pure concentration.   The docking of Las B elastase and Las A alkaline protease with these parameters is achieved for the first time in this study, and no other investigations in this context have been uncovered.

The effects of key components in Rosmarinus officinalis essential oil on the Las B elastase and Las A alkaline protease responsible for Pseudomonas aeruginosa's multidrug resistance capacity were demonstrated in this study.

 We propose association of salicylic acid with Eucalyptol, camphor, alpha pinene and borneol   as a new treatment against multidrug resistant Pseudomonas aeruginosa.