Synthesis Of Silver Nanoparticles And Standardization Of Dose/Concentration Against Selected Plant Pathogenic Fungal Species

Abstract

Bio silver nanoparticles were generated in a lab using Bacillus pseudomycoides MT32 supernatant, and their optimal growing conditions were investigated. The manufactured nanoparticles were characterized by a battery of seven cutting-edge analyzers. The absorption peak in the UV-visible spectra was located around 420 nm. According to results from Transmission Electron Microscopy (TEM), the generated SNPs typically had a diameter of 25 to 43 nm. The particles, as determined by powder X-ray diffraction (XRD), are crystalline, have a spherical shape, and vary in size from 32 to 86 nm. Based on in vitro antifungal testing, the synthesized SNPs had the potential to inhibit, to varying degrees, eleven economically important plant pathogens. The MIC and MFC values reported for these SNPs ranged from 70 to 90 and 75 to 100 g/l, respectively, however these values varied depending on the fungus utilized. Bacillus pseudomycoides MT32's SNPs have antifungal action, which may help with a variety of issues related to agricultural productivity and animal nutrition. Plants are being considered as a viable and inexpensive solution for the biological generation of silver nanoparticles. At 100 ppm silver nanoparticles, C. gloeosporioides did not grow in vitro, although it expanded by 44.50 1.14 mm in the absence of the nanoparticles.