Anticancer Capability of Go-Ark With Plectranthus Amboinicus Against Hepatocellular Carcinoma

Abstract

Cancer is a life threatening disease and an emerging public health issue that affects humans worldwide.  The main objective of the present investigation is to detect the effects of Cow ark, synergistic with ethanolic leaves extract of Plectranthus amboinicus on Diethylnitrosamine (DEN) and 2-Acetylaminofluorene (AAF), stimulated  hepato  carcinogenesis. Cow ark and 5% Ethanolic extract of P. amboinicus were systematically prepared. Identification of in situ  DNA fragmentation was performed by TUNEL assay to explore apoptogenic characteristics of distillate with P.amboinicus  leaves extract.  Apoptotic study observed that a maximum number of apoptotic cells were detected in the liver cells belong to (AB) group, when compared with other tested groups.  The Go-ark with P.amboinicus extract also  showed chemo preventive activities by curing hepatocarcinogenesis activated by DEN/AAF carcinogens. This study proved to be a promising anti-cancer effect of distillate and P. amboinicus extract synergistically in increasing  programmed  cell deaths.