Chemical Mutants Enable The Photo Responsive Synthesis Of Hyaluronic Acid From Streptococcus Equi.

Abstract

Hyaluronic acid (HA) production via traditional methods is labor-intensive and time-consuming since the raw product, which is often generated from animals or microorganisms through fermentation, must be thoroughly purified. The purpose of this research is to find a new and better approach to HA stability production. In order to biosynthesize metal nanoparticles, Streptococcus equi (S. equi) bacteria are exposed to low-intensity ultraviolet (UV) light in the presence of the chemical mutagenic agent ethyl methanesulfonate (EMS) and certain amino acids (L-glutamic acid, L-aspartic acid, L-arginine, L-glycine, and L-lysine). A substantial rise in HA production is seen by the results. After four minutes of exposure to UV light (254nm), 25g/mL of EMS, and 25g/mL of amino acids (glutamic acid and lysine), the bacterial HA output increased from 0.045g/L in the control condition to 0.25g/L and 0.27g/L, respectively. The HA
harvest increased by 5.55 times and by 6 times under these circumstances, respectively. The synthesized HA was very close to the reference sample in terms of molecular mass and chemical analysis. Additionally, the produced HA was used as a capping agent in the biosynthesis of silver (13.02.9nm), gold (21.81.9nm), and ultra-small zero-valent iron (4.10.3nm) nanoparticles. The process is not only useful for the synthesis of different nanomaterials, but also for the manufacture of HA, which appears to be quite successful and
effective.