Oil Production And Biological Activities For Active Compounds From Helianthus Annuus Seed Oil As Important Source In Biofuel And Traditional Medicine

Abstract

In the present study, Fresh seeds of Helianthus annuus were collected during summer season and were dried in the sunlight for 24 hours. The dried seeds were kept in the oil machine and the oil was extracted by heat press method (at 160 C). Around 45ml of the oil was extracted from 100 gm of seeds. About 45% seed oil was obtained from fresh seeds of sunflower. Extracted microbial oil with Helianthus annuus seed waste material was been used for isolation of oil. The seed waste with Saccharomyces cerevisiae was kept for reaction for one week and biofuel was extracted in the present work. The oil from Helianthus annuus seed oil contains Protein, Carbohydrate, amino acids, Terpenoids, Cholesterol, Quinones, flavonoids and Cardiac glycosides. The seed oil has shown little activity with Candida, Enterococcus, Pseudomonas and Staphylococcus. Less antimicrobial activity was observed with Petroleum ether or Ethanol dissolved with microbial oil (50:50).The proteinases like Trypsin and Proteinase K has shown gelatin degradation indicates the proteases making positive effect with gelatin degradation and protein degrading activity. The oil with proteinase has not shown gelatin degradation indicates the oil mix with proteins making negative effect with gelatin degradation. Caffeic acid, Chalcone, Chlorogenic acid, Neoflavonoid and Quinic acid from Helianthus annuus seed oil are selected as ligands. Chlorogenic acid has shown good binding activity showing good antimicrobial activity compared with Caffeic acid, Chalcone, Neoflavonoid and Quinic acid.