Anatomical, Histometrical, And Histochemical Comparative Study Between Stomach Of Ring Necked Parakeet - Psittacula Krameri (Scopoli, 1769) And Black Shouldered Kite - Elanus Caeruleus (Desfontaines, 1789

Abstract

The present study aims to investigate the anatomical, histometrical, and histochemical aspects of the stomach in two species of birds, Psittacula krameri (Ring-necked parakeet) and Elanus caeruleus (Black-shouldered kite). The stomach tissues in the two species were stained with Hematoxylin and Eosin for general histological study and Periodic Acid Schiff (PAS)-Alcian blue (AB) pH 2.5 for histochemical study. An automated analysis system was used for histometrical study. The stomach in two species of birds was divided into proventriculus and ventriculus (gizzard); in the parakeet, proventriculus appeared as fusiform shape and the isthmus separated the gizzard from proventriculus, while in the kite, the proventriculus was pear shape, and the isthmus was absent. In the parakeet, the gizzard was a spherical shape with a thick koilin layer, while the kite has pear shape gizzard with a thin koilin layer. The mucosa was possessed simple tubular glands which were larger and more in the kite; the submucosa was rich with compound tubular glands which were longer and wider in the kite. The histochemical results showed a positive reaction of epithelial cells and simple tubular glands with PAS-AB stains; the koilin and deep gastric glands showed a positive reaction with the PAS stain only.