Antioxidant And Antiulcer Activity Of Phytoconstituents Isolated From Cocinnia Grandis And Diplocyclos Palmatus Fruit

Abstract

Cocinnia grandis and Diplocyclos palmatus family (cucurbitacea) both contains Phytochemical compounds like carbohydrates, steroids, glycosides, flavonoids, alkaloids, andTannins. The secondary metabolites flavonoid and phenolic component.phytochemicals Scavange the free radical  and confirm to their antioxidant activity by DPPH method, hydroxyl radical, ferric radical  scavenging Method and To  Determine  higher the  absobance higher is the potential of the Compound. The Isolated  active chemicals using column chromatography utilizing the gradient elusion technique with the mobile phase n-hexane, ethyl acetate, and ethanol solvent . Four compounds with Rf values of 0.78, 0.69, 0.59, and 0.8 were recovered from Ethyle extract of cocinnia grandis  ( EECG) and given the names CG-1, CG-2, CG- 3, and CG-4.with mobile phases of n-hexane: chloroform and chloroform: methanol, phytoconstituents from Ethyle extract of Diplocyclos palmatus (EEDP) were successfully isolated Three compounds with Rf values of 0.86, 0.81 and 0.6. Acute oral toxicity was conducted in accordance with OECD Directive No. 425 (up and down technique). Using Aspirin plus pylorus ligationd ulcer model, and, isolated compounds from both plants were evaluated at the dosage levels of 20 mg/kg, 10 mg/kg, and 5 mg/kg for antiulcer action. Maximum antiulcer activity was seen for CG-3 and DP-3 at a dosage of 20 mg/kg. Rhamnocitrin, an isolated chemical from Cocinniagrandis fruits, and kaempferol, an isolated compound from Diplocyclos palmatus fruits, both showed notable in vivo antiulcer action by lowering ulcer index, increasing stomach pH, and decreasing ulcer volume. H+ K+ ATPase inhibitory action of CG-3(Rhamnocitrin) supported in vivo antiulcer research. From DP- 3 (kaempferol), which was evaluated using in vitro and molecular docking experiments.Using Molegro Virtual Docker (MVD) (MVD-201,6.0), computer assisted molecular docking simulation investigations were conducted. The results indicated that rhamnocitrin and kaempferol demonstrated excellent contact with low binding energy in the enzyme active pocket through non covalent interaction. As compared to the reference medication Omeprazole, it was shown that CG-3 (Rhamnocitrin) and DP-3 (Kaempferol) strongly inhibited the H+K+ ATPase in a dose-dependent manner.