"Revolutionizing The Enhancement Of Nutritional Conditions In Bioprocessing To Achieve Peak Keratinase Output By Grass Bacillus RAS04 In A Sequential Approach."

Abstract

A keratinase is an extracellular protease capable of efficiently hydrolysing hair and spine keratin into essential amino acids. It has important applications in animal feed as well as slow nitrogen-releasing fertilizer. Extensive keratinous waste soil screened for alkaline proteases and among them selected potent isolates based on REA. Further analysis of keratinase producers using feather degrading efficiency in the basal salt medium. An efficient keratinase analysis of morphology, culture, and biochemistry coupled with 16S rRNA sequencing was used to select and identify the producer as a Grass Bacillus RAS04. An optimization strategy was formulated utilizing “Plackett-Burman design (PBD) and Central Composite Design-Response Surface Methodology (CCD-RSM)” for modulation of nutritional parameters in the bioprocess. The intentionbe to maximize the creation of keratinase from Grass bacillus RAS04 (OQ519653.1). PBD was used to screen out eight variables involved in the processing process.Several factors (such as yeast extract, glucose, feather meal, and K2HPO4) In order to further optimize the variables found to be important to keratinase production, the RSM was fitted with a central composite design. According to the results, 72 hours of incubation in a modified basal salt medium with glucose 0.278%, yeast extract 0.695%, feather meal 0.975%, and K₂HPO₄ 0.121% resulted in positive results, keratinase enzyme activity was 234.41 units/mL, a 2.38-fold increasein activity over the basal medium