Investigations On The Anti-Neoplastic Ability Of Polyscias Fruticosa (L) Harms Root Cunps On Neuroblastoma Cell Lines

Abstract

 The current study focuses on investigating the acute toxicity and anti-neoplastic properties of copper nanoparticles (Pfr CuNP) derived from the root saponin extract (Pfrs) of Polyscias fruticosa. Zeta potential, UV analysis, SEM examinations, and particle size measurement were used to assess the prepared copper nanoparticles. P. fruticosa, which grows in India is a member of the Araliaceae family like ginseng, has high concentration of triterpenoid saponins in its roots and leaves. The bioactivities of ginseng demonstrate many medicinal qualities of this family, which includes immunostimulant, antioxidant, and adaptogenic activities. 

 Based on the SEM data and the typical zeta potential values, the generated copper nanoparticles were found to lie within the nanoparticle range of 100-200 nm. UV λmax was observed at 570 nm for the prepared PfrCuNP. In the Brine Shrimp Assay Method, the LC50 values for PfrCuNP and Pfrs were analysed as 524 mcg/ml and more than 1000 mcg/ml, respectively. Neuroblastoma cell lines were used to screen anti-neoplastic activity, by the MTT assay method. The anticancer activity experiments indicated that PfrCuNP at 125 mcg/ml concentration showed 55.45% cytotoxicity while the reference standard etoposide showed 68.34% cytotoxicity at 50 mcg/ml concentration.