In vitro Cytotoxic studies of Adhatoda vasica leaves on human lung adenocarcinoma cell line (A549)

Abstract

Justicia adhatoda L Whole part methanolic extract (JAWME) is one of the traditional plants with well-known medicinal values prescribed widely to treat many diseases. As globally, we look towards the use of non-toxic plant based products with conventional medicinal usage and the developing countries depends on herbal medicines for their primary health care needs. The aim of this study is to explore the in vitro cytotoxicity of the methanol extract of Justicia adhatoda L Whole part (JAWME) on human lung adenocarcinoma cell line (A549) using MTT assays. The results show a significant inhibition percentage on human lung adenocarcinoma cell line (A549) was observed in both assays. In MTT assay the IC50 values was 176.2µg/ml on methanol extracts respectively. Thus, both MTT assays can be used for cytotoxic screening of leaves of JAWME towards the development of modern drug.