Transgenic Black Mice Induction model for Human Leucoderma

Abstract

There has been little research evaluating the efficacy and safety of 4 hydroxyl anisole + TRA (4 HA + TRA) in an animal model for Leucoderma.To test the  first model of leucoderma to induce epidermal depigmentation, an attempt has been made to achieve an animal model using transgenic mice 4HA+ TRA applications of 3 and 5 %   produced depigmentation, but depigmentation was found to be better in 5 %.  The inducted skin in the transgenic mice was fixed in 10 % neutral formalin and processed by the routine histological technique using F/M methods for the presence/absence of melanin. Good compliance, exposure, and rising cumulative dose were all linked to a better response to therapy, which was statistically significant (P < 0.01). In addition, there were just minor side effects. During follow-up, only half of the animals had depigmentation at the sites.  Our research shows that 4HA combined with TRA is an effective model for Leucoderma, with good depigmentation of the epidermis occurs within 3-4 weeks in a white patchy pattern similar to patients with vitiligo.