Molecular characterization of Acinetobacter baumannii isolated from patients with respiratory tract infections

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Noor Flayyih Hasan , Thanaa Rasheed Abdulrahman , Jabbar R. Zangoor

Abstract

This study aimed for molecular characterization of Acinetobacter baumannii isolated from patients with respiratory tract infectionsfrom clinical specimens includes , sputum , lower respirayory secretion of patients admitted to Medical Teaching City, and Al-Yarmouk Teaching Hospital in Baghdad, Al-Hussein teaching hospital in Karbala ,with study the rate of biofilm formation in Acinetobacter baumannii, in addition to investigate theto determine the frequency of responsible virulence genes. during a period from the first of October 2020 to the end of May 2021.The predominant symptom was Chest pain encountered in patients (22.45%). Ranking in the lowest order Smoking is which was seen in patients (9.80%).In present study, out of 16 isolates there were (50%) possessed RecA gene, Sur A gene (62.50%), PAI gene (56.20%) ,Fim H gene (81.20%) respectively.By PCR amplifiction, the present results showed that, the amplification products of sequences of RecA gene, ITS gene ,Sur A gene PAI gene and Fim H gene in chromosomal DNA were of size 425 bp 208bp,241bp,930bp and 508bp respectively .In the current study, the distribution of genes in Acinetobacterbaumannii clinical isolates showed that, , the highly percentage gene Fim H was identified in all isolates 20(80%),followed SurA 16(64%), ITS 14(56%) ,RecA Gene and PAI 12(48%)genes respectively.
In Conclusion, In Iraqi hospitals, Acinetobacter baumannii was the most common bacterium found in patients with respiratory illnesses.

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