Molecular characterization of Acinetobacter baumannii isolated from patients with respiratory tract infections

Abstract

This study aimed for molecular characterization of Acinetobacter baumannii isolated from patients with respiratory tract infectionsfrom clinical specimens includes , sputum , lower respirayory secretion of patients admitted to Medical Teaching City, and Al-Yarmouk Teaching Hospital in Baghdad, Al-Hussein teaching hospital in Karbala ,with study the rate of biofilm formation in Acinetobacter baumannii, in addition to investigate theto determine the frequency of responsible virulence genes. during a period from the first of October 2020 to the end of May 2021.The predominant symptom was Chest pain encountered in patients (22.45%). Ranking in the lowest order Smoking is which was seen in patients (9.80%).In present study, out of 16 isolates there were (50%) possessed RecA gene, Sur A gene (62.50%), PAI gene (56.20%) ,Fim H gene (81.20%) respectively.By PCR amplifiction, the present results showed that, the amplification products of sequences of RecA gene, ITS gene ,Sur A gene PAI gene and Fim H gene in chromosomal DNA were of size 425 bp 208bp,241bp,930bp and 508bp respectively .In the current study, the distribution of genes in Acinetobacterbaumannii clinical isolates showed that, , the highly percentage gene Fim H was identified in all isolates 20(80%),followed SurA 16(64%), ITS 14(56%) ,RecA Gene and PAI 12(48%)genes respectively.
In Conclusion, In Iraqi hospitals, Acinetobacter baumannii was the most common bacterium found in patients with respiratory illnesses.